Skills

8

  • General Laboratory Procedures: Construction of electrodes, drivers, optical ferrule implants, intravenous catheters, and optical cables.
  • Molecular Biology: bulk RNA extractions, single nuclei isolation, single nuclei RNA sequencing via 10x (GEM generation, barcoding, cleanup, cDNA amplification, and library construction).
  • Histology: Immunohistochemistry, in situ hybridization using RNAscope, Nissl staining, microtome slicing, cryostat slicing, tissue mounting, cover slipping, light microscopy, & fluorescent microscopy.
  • In vivo skills: Animal husbandry, animal handling, intraperitoneal injections, subcutaneous injections, transcardial perfusions, extractions, & animal euthanasia.
  • Surgical Procedures: Stereotaxic surgery in mouse and rat, intravenous catheter implantation for self administration, neurotoxic lesions, viral vector injections, optogenetic ferrule implants, microdrivable electrode bundle implants, and headcap assembly for all implant surgeries.
  • Behavioral Testing: Awake behaving electrophysiology, awake behaving optogenetics, conditioned taste aversion, oral and intravenous self administration, chronic intermittent access paradigms, pavlovian fear conditioning, fear incubation, reversal learning, go/no-go, & omission contingency tasks.
  • Computer Software Experience: MedPC IV, Deep Lab Cut, R, MatLab, SPSS, Statistica, CellSens Standard, ZEISS ZEN microscope software, ImageJ, NeuroExplorer, Offline Sorter, Plexon Omniplex, Microsoft Office Suite (powerpoint, excel, & word), & Adobe Creative Cloud (Photoshop, Illustrator, & Acrobat).
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